SCMV      GTGGTGCTAGTGA.CA.GTGACATTCTGGCTCAGGCTGTCAATCAAGCTGGTATTGACCATAGCTCAGCAGGTACCACCATCACCACCCCATCCA     3358
           | | | | | | | |  | | |  | |  | | | | | | |  | | | |  | | | | | | | |  | | | | | | |  | | | | | | | |  | | | | | | | | | | | | | | | | | |  | | | | | | | | | | | | | | | | | | | |

Stealth   TTGGTGCTAATGA.CA.GTGACATACTGGGTCAGGCTGCCAATCAACCTGGTATTCACCATAGCTCAGCAGGTATCACCATCACCACCCCATCCA       93
           |   |   |  |   |  | |  | | | | | | |  | |  |   | | | | | | |  | | | | | |   |  | | | | |   |        | |   | |  | | |  | | | |   |  | | | |  |    |  |

HCMV      CTTCTTATCACCATCAGGTGACATCCTCGCCCAGGCTGTCAATCATGCCGGTATCGA......TTC..CA.GTAGCACCGGCCCCACGCTGACAA   170782


S    TCTTCACCACCACCACTGCTCCCAGTACCCCCC..AAGGTGTTGTTACTCAGCCTGAGAGTCAGCCCATCCCAC.CACTTGTTT...GCAATCCTGAAAC     3452
     | |  | | | | | | | | | | | | | | | | | | | | | |  | | | | | | |   | | |   |  | | | | | | | | | |  |  | | | | | | | | | | |  | |  | |  |  | | |  | | | || |    | | | | | | | | | | | | |

St   TCCTCACCACCACCACTGCTCCCAGCACCCCCC..AAGTCGCTGTTACTCAGTCAGAGAGTCAGCCAATGCCGC.CACCTGTTT...GCAATCCTGAAAC      187
      |   | | |   |    | |   |  |   | | | |  | | | |  |   | |    | |  |    |  | |   | | |  |    |  |   |  |   |  |  |   | |        | | |   |    |   |

H    CC..CACT.CTTGCAGCGTTAGCAGCGCCCCTCTTAACAAGCCGACCCCCA.CCAGCGTCGCGGTTACTAACACTCCTCTCCCCGGGGCATCCGCTACTC   170686

                              
S    CC.TGTTCATCCCACGTAAGAAATCCCGGAAGACAGACTGCCCCACCAAGATCATTATTAAACCACCCGTGCCTCCCACGTCCACCATGAT.CCCAGCAT     3550
     | |  | | | | | | | | | | | | | | | | |  | | | | | | | | | | | | | | | | | | | | | |  | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |  | | | | | | | |

St   CC.TGTTCATCCCACGTAAGGAATCCCGGAAGACAGACTGCCCTACCAAGATCATTATTAAACCACCCGTGCCTCCCACGTCCACCATGAT.CCCAGCAT      285
     | |   |  | | |  | | |  | | | | | |  | |  |  | |  | |  | |      |  | | |   | | | |  |  | | | | | | | | | | |  | | | | | | | | | | | |  | |  |  |  | | | |  |  | | |   | | |

H    CCGAGCTCAGCCCGCGTAAGAAACCGCGCAAAACCACGCGTCCTTTCAAGGTGATTATTAAACCGCCCGTGCCTCCCGCGCCTATCATGCTGCCCCTCAT   170586

                              EcoRI
S    CCCAGATTAAGAAA..GAGCCTGAGGAATTCTTCAAGCTCCAGTACAAAGACCAGGACATCCAACCCACCTCTGGATGTATTGTGATCTCAGACAGTGAA     3648
     | | | | | | | | | | | |  |   | | | | | | | | | | | | | | | | | | | | | | | | | |  | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |

St   CCCAGATTAAGATA..GAGCCTGAGGAATTCTTCAAGCTCCAATACAAAGACCAGGACATCCAACCCACCTCTGGATGTATTGTGATCTCAGACAGTGAA      285
     |   |  |   | | | |  |    | | | |  | | |      | | |  |    | | | |  | | |     | |  | |    | | |  |     | | |  |  | |  | | | | |  | | | | | | | |  | |  | |

H    CAAACAGGAAGACATCAAGCCCGAGCCCGACTTTACCATCCAGTACCGCAACAAGATTATCGA...TACCGCCGGCTGTATCGTGATCTCTGATAGC...   170492


S    GAGGAAGAAGACACT.CAGACTCTGATTCCCAC..AGCTTCC...TCCTCCTCTTCCTCAGAGAACCAGGGTGTGCAGCTGACAATGACCAC.CCCAGGC     3741
     | | | | | | | | | | | | | | |  | | | | | | | | | | | | | | | | |   | | |  | | |    | |  | | | |        | | |  | | | | | | | | | | | | | | | | | | | | | | | | | | | | |  | | | | | | |

St   GAGGAAGAAGACACT.CAGACTCTGATTCCCAC..AGCCTCC...TCTTCCT......TAGATAACCAGGGTGTGCAGCTGACAATGACCAC.CCCAGGC      470
     | | | | | | | | |  |    |   | | |     | |   | | |  |    | |   | |    | | | | | |          |   |  |    | |    | |  | |  |     | | | | |  |  | | | |  | |

H    GAGGAAGAACAGGGTGAAGAAGTCGAAACCCGCGGTGCTACCGCGTCTTCCCCTTCCACCGGCAGC...GGCACGCCGC.G..AGTGACCTCTCCCACGC   170398


S    AGTGGATCAGTGGGCAAAATGTCTGTGGAGAGTTCCTCATCCTCCAGCAGCGAGTCAGAGTGCTG.TGAAGAATGTGGACTTTCATCTCCCAGTACGTTG     3840
     | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |  | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |

St   AGTGGATCAGTGGGCAAAATGTCTGTGGAGAGTTCCTCATCCTCCAGCAGCGAGTCAGAGTGCTG.TGAAGAATGTGGACTTTCATCTCCCAGTACGTTG      569
     |    |  | |      |    | |    |   |       | | | |   | | | |     |   | |   |  |  | |  | |   |         |  |      | | |  | |   | |  |  |   |  |

H    ACCCGCTCTCCCAGATGAACCACCCT..CCTCTTCCCGATCCCTTGG..GCCGGCCCGA.TGAAGATAGTTCCTCTTCGTCTTCCTCCTCCTGCAGTTCG   170303


S    GCATC...TCCAGTGTCCCCCATTCCACCACCCCCACCAGCACCCGTGAT.GCCCAGCACCTCTGGTCGCAAGC..CCAAGGGTCCCAAGACCAAGACCA     3934
     | | | | |     | |  | | | | |  | | |  | | | | | | | | | | | | | | | | | | | | | | | | | | | |  | | | | | | | | | | | | | | | |   | | | | |   | | | | | | | | | | | | | | | | | |  | | | | |

St   GCATC...CCCGGTGTCTCCCCTTCCACCACCCCCACCAGCACCCGTGAT.GCCCAGCACCTCTGGTGCCAAGC..CCAAGGGTCCCAAGACCATGACCA      663
     | |  | |    |  | | |  |   |     |  |  |   |      |    | | |  |  | | |    |    | | | |  |   | |     |  | | |   | | |  | | |   |    |     | |

H    GCTTCGGACTCGGAGAGT.GAGTCCGAGGAGATGAAAT.GCAGCAGTGGCGGAGGAGCATCCGTGACCTCGAGCCACCATGGGCGCGGCGGTTTTGGTGG   170205

Fig.1 Alignment using FASTA of the nucleotide sequences of SCMV (S), stealth virus-1 (St) and HCMV (H). The regions shown for SCMV and HCMV start two nucleotides prior to the beginning of the 5th exon of the MIE gene (GenBank U18245 and X17403). The sequence of stealth virus-1 represents the composite read-out using the T7 sequencing promoter on Eco RI clones 3B561 and 3B615, respectively. The Eco RI site is indicated. Nucleotide numbers are shown at the end of each line.